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Respiration Rate Lab
Name:
Date:
Period:
Purpose:
To determine the effect of temperature variation on the respiration rate in fish.
Procedure:
The respiration rate in fish can be determined by counting respiration cycles a fish makes in one minute. A respiration cycle starts when the fish opens its mouth and opercula to take in water. Water moves across the gills and out the opercula. When the opercula both close the cycle is complete. Record the breaths in the data table below. Use the slider to change the temperature.
Data:
Temperature °C
Respiration Rate
15°
25°
35°
Analysis:
Graph your results.
Respiration Rate
90
80
70
60
50
40
30
20
10
0
15°
25°
35°
45°
Temperature °C
Conclusion:
1. Describe the relationship between temperature and respiration rate.
2. What would you expect the breathing rate to be at 20 °C?
3. What would you expect the breathing rate to be at 40 °C?
4. What temperature would cause the fish to breathe at a rate of 90 breaths/minute?
Capillary Blood Flow Lab
Name:
Date:
Period:
Purpose:
To determine the effect of various drugs on the flow rate of blood in capillaries.
Procedure:
Count the number of times the pink marker flows by in one minute to determine the normal flow rate. Then determine how each of the four drugs affect the flow rate. Select a drug by dragging a cotton ball to the fish's operculum. Record your data in the table below. Drag the cotton ball with just water to the fish's operculum to restore the normal flow rate.
Data:
Drug
Heartbeats per
minute
Normal
Nicotine
Alcohol
Caffeine
Adrenaline
Analysis:
Graph your results.
Flow Rate/minute
45
40
35
30
25
20
15
10
5
0
Normal
Nicotine
Alcohol
Caffeine
Adrenaline
Drug
Conclusion:
1. How did the different drugs affect the rate of blood flow in the fish?


2. A stimulant is a drug that increases physiological activity, such as blood flow rate. Which drug(s) would be considered a stimulant?

3. A depressant is a drug that decreases physiological activity, such as blood flow rate. Which drug(s) would
be considered a depressant?
Dissolved Oxygen Lab
Name:
Date:
Period:
Purpose:
To determine the effect of temperature variation on the amount of dissolved oxygen in water.
Procedure:
Fish and other aquatic animals depend on the dissolved oxygen present in the water. The beaker contains water that has its dissolved oxygen fixed and it is ready to be titrated. Use the slider to select the temperature of the sample to be tested. Next click on the bottle of sodium thiosulfate (Na2S2O3) to activate the dropper. Add drops to the beaker until it becomes completely colorless. The number of drops is equal to the ml/liter of dissolved oxygen in the water. Record the amount of dissolved oxygen for each temperature.
Data:
Temperature °C
ml/L of dissolved O2
(Drops of Na2S2O3)
15°
25°
35°
Analysis:
Graph your results.
Dissolved Oxygen ml/Liter
9
8
7
6
5
4
3
2
1
0
15°
25°
35°
45°
Temperature °C
Conclusion:
1. Describe the relationship between temperature and the amount of dissolved oxygen that water contains.
2. What would you expect the dissolved oxygen content to be at 20° C?
3. What would you expect the dissolved oxygen content to be at 45° C?
4. What temperature would result in a dissolved oxygen content of 8ml / liter?
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